[RT-PCR for the determinationof the type of influenza virus circulating in the population].
Identifieur interne : 000662 ( Main/Exploration ); précédent : 000661; suivant : 000663[RT-PCR for the determinationof the type of influenza virus circulating in the population].
Auteurs : R. Cisterna ; E. MeabeSource :
- Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia [ 0214-3429 ] ; 2000.
Descripteurs français
- KwdFr :
- ARN viral (biosynthèse), ARN viral (génétique), Espagne (MeSH), Grippe humaine (virologie), Humains (MeSH), Inspiration (MeSH), Lignée cellulaire (MeSH), Orthomyxoviridae (classification), Orthomyxoviridae (génétique), Protéines virales (composition chimique), RT-PCR (MeSH), Réaction de polymérisation en chaîne (MeSH), Technique d'immunofluorescence (MeSH), Virus de la grippe A (classification), Virus de la grippe A (génétique), Virus influenza B (classification), Virus influenza B (génétique).
- MESH :
- biosynthèse : ARN viral.
- composition chimique : Orthomyxoviridae, Protéines virales, Virus de la grippe A, Virus influenza B.
- génétique : ARN viral, Orthomyxoviridae, Virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine.
- Espagne, Humains, Inspiration, Lignée cellulaire, RT-PCR, Réaction de polymérisation en chaîne, Technique d'immunofluorescence.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Cell Line (MeSH), Fluorescent Antibody Technique (MeSH), Humans (MeSH), Influenza A virus (classification), Influenza A virus (genetics), Influenza B virus (classification), Influenza B virus (genetics), Influenza, Human (virology), Inhalation (MeSH), Orthomyxoviridae (classification), Orthomyxoviridae (genetics), Polymerase Chain Reaction (MeSH), RNA, Viral (biosynthesis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Spain (MeSH), Viral Proteins (chemistry).
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , chemistry : Viral Proteins.
- geographic : Spain.
- classification : Influenza A virus, Influenza B virus, Orthomyxoviridae.
- genetics : Influenza A virus, Influenza B virus, Orthomyxoviridae, RNA, Viral.
- virology : Influenza, Human.
- Cell Line, Fluorescent Antibody Technique, Humans, Inhalation, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the type of flu virus that was circulating mostly in the population of the Basque Country in the 1999-2000 season. In this time period, 124 nasal and pharyngeal aspirations from persons with flu-like symptoms were analyzed. A parallel analysis was carried out using a conventional culture or a shell-vial in MDCK cell line, and immunofluorescence and multiple RT-PCR for the detection of influenza virus and its A and B types. Of the samples studied, 57 (45.96%) were positive for type A by the culture method and 64 (51. 61%) by RT-PCR of a genomic region that codifies the non-structural proteins NS1 and NS2. We also developed a rapid-detection method in which the transcription and amplification are carried out in a single step, using the same mix of transcription-amplification. Based on the results we can conclude that RT-PCR is a very sensitive method for the detection of the flu virus; we believe that its sensitivity, combined with its rapidity, makes it ideal for the detection of these respiratory viruses and the analysis of strains circulating in the population.
PubMed: 11086279
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Meabe</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11086279</idno><idno type="pmid">11086279</idno><idno type="wicri:Area/Main/Corpus">00665</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">00665</idno><idno type="wicri:Area/Main/Curation">000665</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000665</idno><idno type="wicri:Area/Main/Exploration">000665</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[RT-PCR for the determinationof the type of influenza virus circulating in the population].</title><author><name sortKey="Cisterna, R" sort="Cisterna, R" uniqKey="Cisterna R" first="R" last="Cisterna">R. Cisterna</name><affiliation><nlm:affiliation>Servicio de Microbiología Clínica, Hospital de Basurto, Facultad de Medicina, Universidad del País Vasco.</nlm:affiliation><wicri:noCountry code="subField">Universidad del País Vasco</wicri:noCountry></affiliation></author><author><name sortKey="Meabe, E" sort="Meabe, E" uniqKey="Meabe E" first="E" last="Meabe">E. 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In this time period, 124 nasal and pharyngeal aspirations from persons with flu-like symptoms were analyzed. A parallel analysis was carried out using a conventional culture or a shell-vial in MDCK cell line, and immunofluorescence and multiple RT-PCR for the detection of influenza virus and its A and B types. Of the samples studied, 57 (45.96%) were positive for type A by the culture method and 64 (51. 61%) by RT-PCR of a genomic region that codifies the non-structural proteins NS1 and NS2. We also developed a rapid-detection method in which the transcription and amplification are carried out in a single step, using the same mix of transcription-amplification. Based on the results we can conclude that RT-PCR is a very sensitive method for the detection of the flu virus; we believe that its sensitivity, combined with its rapidity, makes it ideal for the detection of these respiratory viruses and the analysis of strains circulating in the population.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11086279</PMID><DateCompleted><Year>2001</Year><Month>01</Month><Day>09</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0214-3429</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>3</Issue><PubDate><Year>2000</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia</Title><ISOAbbreviation>Rev Esp Quimioter</ISOAbbreviation></Journal><ArticleTitle>[RT-PCR for the determinationof the type of influenza virus circulating in the population].</ArticleTitle><Pagination><MedlinePgn>286-90</MedlinePgn></Pagination><Abstract><AbstractText>Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the type of flu virus that was circulating mostly in the population of the Basque Country in the 1999-2000 season. In this time period, 124 nasal and pharyngeal aspirations from persons with flu-like symptoms were analyzed. A parallel analysis was carried out using a conventional culture or a shell-vial in MDCK cell line, and immunofluorescence and multiple RT-PCR for the detection of influenza virus and its A and B types. Of the samples studied, 57 (45.96%) were positive for type A by the culture method and 64 (51. 61%) by RT-PCR of a genomic region that codifies the non-structural proteins NS1 and NS2. We also developed a rapid-detection method in which the transcription and amplification are carried out in a single step, using the same mix of transcription-amplification. Based on the results we can conclude that RT-PCR is a very sensitive method for the detection of the flu virus; we believe that its sensitivity, combined with its rapidity, makes it ideal for the detection of these respiratory viruses and the analysis of strains circulating in the population.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cisterna</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Servicio de Microbiología Clínica, Hospital de Basurto, Facultad de Medicina, Universidad del País Vasco.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meabe</LastName><ForeName>E</ForeName><Initials>E</Initials></Author></AuthorList><Language>spa</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><VernacularTitle>RT-PCR para la determinación del tipode los virus de la gripecirculantes entre la población. Grupo de Estudio de la Gripe de la Sociedad Española de Quimioterapia.</VernacularTitle></Article><MedlineJournalInfo><Country>Spain</Country><MedlineTA>Rev Esp Quimioter</MedlineTA><NlmUniqueID>9108821</NlmUniqueID><ISSNLinking>0214-3429</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001239" MajorTopicYN="N">Inhalation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009975" MajorTopicYN="N">Orthomyxoviridae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="Y">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N" Type="Geographic">Spain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>11</Month><Day>22</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>11</Month><Day>22</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11086279</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Cisterna, R" sort="Cisterna, R" uniqKey="Cisterna R" first="R" last="Cisterna">R. 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